#１. Exosomes

Exsomes are cell-derived extracellular vesicles enveloped by a lipid bilayer. Exosomes are released into the extracellular environment containing a complex cargo of contents derived from original cell, including cytokine, mRNA, miRNA and lipids. Exsomes transport molecules that are important regulators of intracellular information between close and distant cells. Exsome has known to be quite important in maintaining a healthy metabolism.

#2. Characteristics of exosomes in cord blood stem cell-derived conditioned media.

Exosomes in cord blood stem cell-derived conditioned media, are typically 80-160 nm in diameter. We compared the size of exsomes in lipid stem cell-derived conditioned media, the size are 180nm-300nm. ^*1 The results show Exsomes in cord blood stem cell-derived conditioned media are smaller and contained more particles.

*1 ) Exosomes derived from human umbilical cord blood mesenchymal stem cells stimulates rejuvenation of human skin // Biochemical and Biophysical Research Communications 493 (2017) 1102e1108

#3. Skin Penetration of Cord Blood Stem Cell-Derived Exosomes

An experiment using skin tissue confirmed the penetration of cord blood stem cell-derived exosomes into the skin. ^*1

#4. FCJ-EX1

We isolated the exosomes from cord blood stem cell-derived conditioned media and we counted the numbers of isolated exosomes.